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CryoEM: do you have any suggestion to concentrate samples after GRAFIX gradient?

After GRAFIX I am loosing all my sample on amicon filter.

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MaKo
Hello, loosing small quantities on amicon filters is quite common and dependant on the particular protein even huge amounts. Did you try other concentration methods ? Cryoconcentration is in my hands one of the best, but I do not know whether that will hamper your EM work afterwards. Best, Marcel 
Type
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Status
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Bounty
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Created
05/16/2023
Answer Count
1
Vote Count
1
Creator
Stamburr
Tags
Biochemistry